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Antibody used in WB on Human Liver at 0.2-1 ug/ml.
Antibody used in WB on Human Liver at 0.2-1 ug/ml.

DPH4 Antibody

CATALOG NUMBER: 30-874

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DPH4 antibody can be used for detection of DPH4 by ELISA at 1:312500. DPH4 antibody can be used for detection of DPH4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Predicted Molecular Weight:
17 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DPH4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DNAJC24
Additional Names:
DPH4, JJJ3, ZCSL3, DPH4
Protein Accession Number:
NP_859057
Protein Gi Number:
89111951
Ncbi Gene Id Number:
120526
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2.Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).
Background Reference 1:
Liu, S., (2004) Mol. Cell. Biol. 24 (21), 9487-9497.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-874

List Size:
100 ul

List Price:

$519.00

Datasheet
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