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46-738 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-738 (3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-738 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

DPM1 Antibody

CATALOG NUMBER: 46-738

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: RELEVRSPRQNKYS-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 28kDa band observed in Human Liver lysates (calculated MW of 29.6kDa according to NP_003850.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DPM1
Additional Names:
DPM1, dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit, CDGIE, MPDS, dolichol monophosphate mannose synthase, dolichyl-phosphate mannosyltransferase polypeptide 1
Protein Accession Number:
NP_003850.1
Protein Gi Number:
4503363
Ncbi Gene Id Number:
8813
Background Reference 1:
Ashida H, Maeda Y, Kinoshita T. DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3. J Biol Chem. 2006 Jan 13;281(2):896-904.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-738

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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