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Western blot analysis in HL-60 cell line lysates (35ug/lane).
DPP3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of DPP3 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in HL-60 cell line lysates (35ug/lane).

DPP3 Antibody

CATALOG NUMBER: 55-722

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This DPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 583-612 amino acids from the C-terminal region of human DPP3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DPP3
Additional Names:
Dipeptidyl peptidase 3, Dipeptidyl aminopeptidase III, Dipeptidyl arylamidase III, Dipeptidyl peptidase III, DPP III, Enkephalinase B, DPP3
Protein Accession Number:
Q9NY33
Protein Gi Number:
20532389
Ncbi Gene Id Number:
10072
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that is a member of the M49family of metallopeptidases. This cytoplasmic protein binds asingle zinc ion with its zinc-binding motif (HELLGH) and haspost-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Prodipeptides from the N-termini of proteins. Increased activity ofthis protein is associated with endometrial and ovarian cancers.Alternate transcriptional splice variants have been characterized.
Background Reference 1:
Shukla, A.A., et al. FEBS J. 277(8):1861-1875(2010)
Background Reference 2:
Salopek-Sondi, B., et al. Biol. Chem. 389(2):163-167(2008)
Background Reference 3:
Gevaert, K., et al. Proteomics 5(14):3589-3599(2005)
Background Reference 4:
Abramic, M., et al. Int. J. Biochem. Cell Biol. 36(3):434-446(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-722

List Size:
400 ul

List Price:

$495.00

Datasheet
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