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Western blot analysis of extracts of various cell lines, using DPP8 antibody (22-710) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of A549 cells using DPP8 antibody (22-710). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using DPP8 antibody (22-710) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

DPP8 Antibody

CATALOG NUMBER: 22-710

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 10-230 of human DPP8 (NP_932065.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Positive Control 1:
HL-60
Positive Control 2:
MCF7
Positive Control 3:
NCI-H460
Positive Control 4:
HeLa
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse lung
Predicted Molecular Weight:
Observed: 103kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DPP8
Additional Names:
DPP8, Dipeptidyl-peptidase 8, DPRP1, Dipeptidyl peptidase VIII, Dipeptidylpeptidase 8, DPRP-1, Dipeptidyl peptidase 8, MSTP141, Prolyl dipeptidase DPP8, DP8, DPP VIII
Ncbi Gene Id Number:
54878
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-710

List Size:
100 uL

List Price:

$436.00

Datasheet
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