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Western Blot at 1:1000 dilution + human fetal brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of DPYSL3 using rabbit polyclonal DPYSL3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DPYSL3 gene.
DPYSL3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + human fetal brain lysate Lysates/proteins at 20 ug per lane.

DPYSL3 Antibody

CATALOG NUMBER: 57-578

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This DPYSL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 461-490 amino acids from the C-terminal region of human DPYSL3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
62 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DPYSL3
Additional Names:
Dihydropyrimidinase-related protein 3, DRP-3, Collapsin response mediator protein 4, CRMP-4, Unc-33-like phosphoprotein 1, ULIP-1, DPYSL3, CRMP4, DRP3, ULIP, ULIP1
Protein Accession Number:
Q14195
Protein Gi Number:
3122050
Ncbi Gene Id Number:
1809
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).
Background Reference 1:
Okita, K., et al. Cancer Sci. 101(11):2333-2340(2010)
Background Reference 2:
Gao, X., et al. Oncogene 29(32):4555-4566(2010)
Background Reference 3:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 4:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-578

List Size:
400 ul

List Price:

$495.00

Datasheet
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