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Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:1000 dilution.
Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 μg/mL.
Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 μg/mL.
Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 μg/mL.<br><br>Green: DR3 Antibody (1158) <br> Blue: DAPI staining
Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 μg/mL.
Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:1000 dilution.
Trial Size

DR3 Antibody

CATALOG NUMBER: 1158

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
DR3 antibody was raised against a peptide corresponding to amino acids in extracellular domain of human DR3 precusor.
The immunogen is located within amino acids 50 - 100 of DR3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
DR3 antibody can be used for detection of DR3 expression by Western blot at 1 μg/mL. 59 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 17-005 - Jurkat Cell Slide
Predicted Molecular Weight:
59 kDa
Purification:
DR3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DR3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
DR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TNFRSF25
Additional Names:
DR3 Antibody: DR3, TR3, DDR3, LARD, APO-3, TRAMP, WSL-1, WSL-LR, TNFRSF12, APO3, DR3, WSL, WSL1, UNQ455/PRO779, Tumor necrosis factor receptor superfamily member 25, Apo-3
Protein Accession Number:
AAQ88676
Protein Gi Number:
37181738
Ncbi Gene Id Number:
8718
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DR3 Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD1-5. The ligand for this novel death receptor has been defined as TWEAK, also termed Apo3L. DR3 is highly expressed in the tissues enriched in lymphocytes including PBL, thymus and spleen. Like TNFR1, DR3 induces apoptosis and NF-κB activation.
Background Reference 1:
Chinnaiyan AM; O'Rourke K; Yu GL; Lyons RH; Garg M; Duan DR; Xing L; Gentz R; Ni J; Dixit VM. Science, 1996;274:990-2.
Background Reference 2:
Kitson J; Raven T; Jiang YP; Goeddel DV; Giles KM; Pun KT; Grinham CJ; Brown R; Farrow SN. Nature, 1996;384:372-5.
Background Reference 3:
Marsters SA; Sheridan JP; Donahue CJ; Pitti RM; Gray CL; Goddard AD; Bauer KD; Ashkenazi A. Curr Biol, 1996;6:1669-76.
Background Reference 4:
Bodmer JL; Burns K; Schneider P; Hofmann K; Steiner V; Thome M; Bornand T; Hahne M; Schroter M; Becker K; et al. Immunity, 1997;6:79-88.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

1158

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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