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Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse cerebellum lysate Lane 4: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with DRAGON (RGMB) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of DRAGON (RGMB) Antibody in K562 cell line lysates (35ug/lane)
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse cerebellum lysate Lane 4: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.

DRAGON Antibody

CATALOG NUMBER: 57-567

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This DRAGON (RGMB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-406 amino acids from the C-terminal region of human DRAGON (RGMB).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RGMB
Additional Names:
RGM domain family member B, DRG11-responsive axonal guidance and outgrowth of neurite, DRAGON, RGMB
Protein Accession Number:
Q6NW40
Protein Gi Number:
327478562
Ncbi Gene Id Number:
285704
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Repulsive Guidance Molecule B (RGMB) is primarily expressed in the central nervous system. RGM A and B both appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGMA appears in the neuroepithelium, and only RGMB in the peripheral nervous system. During development, both RGMA and B appear also in lung and in limb cartilage, while RGMB has additional expression domains in pancreas.
Background Reference 1:
Schnichels,S., Gene Expr. Patterns(1), 1-11 (2007)
Background Reference 2:
Metzger,M., Dev. Dyn. 234 (1), 169-175 (2005)
Background Reference 3:
Xia,Y., Endocrinology 146 (8), 3614-3621 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-567

List Size:
400 ul

List Price:

$495.00

Datasheet
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