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Western blot analysis of DRAK1 in (A) MOLT4 and (B) A431 whole cell lysates with DRAK1 antibody at 1 μg/mL.
Immunocytochemistry staining of MOLT4 cells using DRAK1 antibody at 2 μg/mL.
Immunofluorescence of DRAK1 in Molt cells with DRAK1 antibody at 20 μg/mL.
Western blot analysis of DRAK1 in (A) MOLT4 and (B) A431 whole cell lysates with DRAK1 antibody at 1 μg/mL.
Trial Size

DRAK1 Antibody

CATALOG NUMBER: 2147

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (73%)
Immunogen:
DRAK1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human DRAK1.
The immunogen is located within the first 50 amino acids of DRAK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
DRAK1 antibody can be used for detection of DRAK1 by Western blot at 1 μg/mL. An approximately 50 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
No cross responses to DRAK2, DAP or ZIP kinases.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 1206 - MOLT4 Cell Lysate
Positive Control 3:
Cat. No. 17-006 - MOLT-4 Cell Slide
Predicted Molecular Weight:
50 kDa
Purification:
DRAK1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DRAK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
DRAK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
STK17A
Additional Names:
DRAK1 Antibody: DRAK1, DRAK1, DAP kinase-related apoptosis-inducing protein kinase 1
Protein Accession Number:
Q9UEE5
Protein Gi Number:
14423930
Ncbi Gene Id Number:
9263
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DRAK1 Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 for DAP kinase-related apoptosis-inducing protein kinases. DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK1 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK1 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.
Background Reference 1:
Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2147

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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