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Western blot analysis of extracts of mouse brain, using DRD3 antibody (16-111) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using DRD3 Antibody (16-111) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DRD3 Antibody (16-111) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using DRD3 Antibody (16-111) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DRD3 antibody (16-111). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of mouse brain, using DRD3 antibody (16-111) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

DRD3 Antibody

CATALOG NUMBER: 16-111

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 213-329 of human DRD3 (NP_000787.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse brain
Predicted Molecular Weight:
Observed: 44kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DRD3
Additional Names:
DRD3, D3 dopamine receptor, D3DR, Dopamine 3 receptor, Dopamine receptor D3, D3nf, Essential tremor 1, ETM1, FET1, D(3) dopamine receptor, Dopamine D3 receptor
Ncbi Gene Id Number:
1814
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the D3 subtype of the five (D1-D5) dopamine receptors. The activity of the D3 subtype receptor is mediated by G proteins which inhibit adenylyl cyclase. This receptor is localized to the limbic areas of the brain, which are associated with cognitive, emotional, and endocrine functions. Genetic variation in this gene may be associated with susceptibility to hereditary essential tremor 1. Alternative splicing of this gene results in transcript variants encoding different isoforms, although some variants may be subject to nonsense-mediated decay (NMD).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-111

List Size:
100 uL

List Price:

$436.00

Datasheet
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