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Western blot analysis of DRGX in rat liver tissue lysate with DRGX antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of DRGX in rat liver cells with DRGX antibody at 20 μg/mL.
Western blot analysis of DRGX in rat liver tissue lysate with DRGX antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

DRGX Antibody

CATALOG NUMBER: 6517

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
DRGX antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human DRGX.
The immunogen is located within the last 50 amino acids of DRGX.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
DRGX antibody can be used for detection of DRGX by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
At least two are known to exist; DRGX antibody is predicted to not cross-react with other Homeodomain proteins.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Purification:
DRGX Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
DRGX Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DRGX antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DRGX
Additional Names:
DRGX Antibody: DRG11, PRRXL1, Dorsal root ganglia homeobox protein, Paired-related homeobox protein-like 1
Protein Accession Number:
NP_001073989
Protein Gi Number:
122937484
Ncbi Gene Id Number:
644168
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DRGX Antibody: DRGX, also known as DRG11 or PRRXL1, is a paired homeodomain transcription factor, and was initially cloned from a differential hybridization screen aimed at identifying dorsal root ganglia (DRG)-specific genes. DRGX is a key regulator of the differentiation of the spinal cord neuronal circuit dedicated to the processing of nociceptive information. DRGX is also important for the formation of the whisker-related somatosensory maps in the principal sensory nucleus of the trigeminal nerve, thalamus and somatosensory cortex.
Background Reference 1:
Saito T, Greenwood A, Sun Q et al. Identification by differential RT-PCR of a novel paired homeodomain protein specifically expressed in sensory neurons and a subset of their CNS targets. Mol. Cell. Neurosci. 1995; 6:280-92.
Background Reference 2:
Chen ZF, Rebelo S, White F et al. The paired homeodomain protein DRG11 is required for the projection of cutaneous sensory afferent fibers to the dorsal spinal cord. Neuron 2001; 31:59-73.
Background Reference 3:
Ding YQ, Yin J, Xu HM, et al. Formation of whisker-related principal sensory nucleus-based lemniscal pathway requires a paired homeodomain transcription factor, Drg11. J. Neurosci. 2003; 23:7246-54
Background Reference 4:
Rebelo S, Lopes C, Lima D, et al. Expression of a Prrxl1 alternative splice variant during the development of the mouse nociceptive system. Int. J. Dev. Biol. 2009; 53:1089-95.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6517

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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