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Western blot analysis of anti (AP7795a) in T47D cell line lysates (35ug/lane)
Flow cytometric analysis of MCF-7 cells using (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of anti (AP7795a) in T47D cell line lysates (35ug/lane)

DSG2 Antibody

CATALOG NUMBER: 63-211

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DSG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-216 amino acids from the N-terminal region of human DSG2.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
122 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DSG2
Additional Names:
Desmoglein-2, Cadherin family member 5, HDGC, DSG2, CDHF5
Protein Accession Number:
Q14126
Protein Gi Number:
148876773
Ncbi Gene Id Number:
1829
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This protein is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in the DSG2 gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10.
Background Reference 1:
Posch,M.G., Mol. Genet. Metab. 95 (1-2), 74-80 (2008)
Background Reference 2:
Yu,C.C., J. Formos. Med. Assoc. 107 (7), 548-558 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-211

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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