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DSS1 Antibody

CATALOG NUMBER: 45-500

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-RAELEKHGYKMETS
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1ug/ml. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size of approx. 8KDa according to NP_006295. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunocytochemistry: Anonymous customer found nuclear staining in HT1080.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SHFM1
Additional Names:
SHFM1, split hand/foot malformation (ectrodactyly) type 1, DSS1, Deleted in split-hand/split-foot 1 region, ECD, SEM1, SHFD1, SHSF1, Shfdg1, candidate for split hand/foot malformation type 1, deleted in split-hand/foot 1, deleted in split-hand/split-foot 1, split hand/foot malformation type 1
Protein Accession Number:
NP_006295.1
Protein Gi Number:
5453640
Ncbi Gene Id Number:
7979
Background Reference 1:
Wei SJ, Trempus CS, Cannon RE, Bortner CD, Tennant RW. Identification of Dss1 as a 12-O-Tetradecanoylphorbol-13-acetate-responsive Gene Expressed in Keratinocyte Progenitor Cells, with Possible Involvement in Early Skin Tumorigenesis. J Biol Chem. 2003 Jan 17;278(3):1758-68.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-500

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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