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Western blot analysis of DSTN Antibody in MCF-7 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with DSTN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DSTN using rabbit polyclonal DSTN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DSTN gene.
Western blot analysis of DSTN Antibody in MCF-7 cell line lysates (35ug/lane)

DSTN Antibody

CATALOG NUMBER: 63-829

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This DSTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the Central region of human DSTN.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
19 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DSTN
Additional Names:
Destrin, Actin-depolymerizing factor, ADF, DSTN, ACTDP, DSN
Protein Accession Number:
P60981
Protein Gi Number:
46577586
Ncbi Gene Id Number:
11034
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DSTN belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This protein encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin).
Background Reference 1:
Estornes,Y., et.al., Int. J. Cancer 121 (10), 2162-2171 (2007)
Background Reference 2:
Mannherz,H.G., et.al., J. Mol. Biol. 366 (3), 745-755 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-829

List Size:
400 ul

List Price:

$495.00

Datasheet
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