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Western blot analysis of DTX1 Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lymph tissue reacted with DTX1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DTX1 Antibody in Hela cell line lysates (35ug/lane)

DTX1 Antibody

CATALOG NUMBER: 63-705

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This DTX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the Central region of human DTX1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
67 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DTX1
Additional Names:
E3 ubiquitin-protein ligase DTX1, 632-, Protein deltex-1, Deltex1, hDTX1, DTX1
Protein Accession Number:
Q86Y01
Protein Gi Number:
37077046
Ncbi Gene Id Number:
1840
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DTX1 was identified as encoding a positive regulator of the Notch-signaling pathway in Drosophila. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity.
Background Reference 1:
Wu,C.,et.al., Proteomics(11), 1775-1785 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-705

List Size:
400 ul

List Price:

$495.00

Datasheet
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