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43-377 (0.1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-377 (0.1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

DUOX1 Antibody

CATALOG NUMBER: 43-377

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CSRSDIETELTPQR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 170kDa band observed in Human Testis lysates (calculated MW of 177kDa according to NP_059130.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 140kDa. This band was successfully blocked by incubation with the immunizing peptide.
Specificity:
Reported variants represent identical protein: NP_787954.1, NP_059130.2. This antibody is not expected to cross-react with DUOX2.
Predicted Molecular Weight:
Approx 170kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DUOX1
Additional Names:
DUOX1, dual oxidase 1, LNOX1, MGC138840, MGC138841, NOXEF1, THOX1, NADPH thyroid oxidase 1, flavoprotein NADPH oxidase, large NOX 1, long NOX 1, nicotinamide adenine dinucleotide phosphate oxidase, thyroid oxidase 1
Protein Accession Number:
NP_059130.2
Protein Gi Number:
20149640
Ncbi Gene Id Number:
53905
Background Reference 1:
Harper RW, Xu C, Eiserich JP, Chen Y, Kao CY, Thai P, Setiadi H, Wu R.Differential regulation of dual NADPH oxidases/peroxidases, Duox1 and Duox2, by Th1 and Th2 cytokines in respiratory tract epithelium.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-377

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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