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Antibody is used in Western blot to detect DUSP1 in 293 cell lysate.
Confocal immunofluorescent analysis of DUSP1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Antibody is used in Western blot to detect DUSP1 in 293 cell lysate.

DUSP1 Antibody

CATALOG NUMBER: 63-488

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This DUSP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-76 amino acids from the N-terminal region of human DUSP1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DUSP1
Additional Names:
Dual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, Mitogen-activated protein kinase phosphatase 1, MAP kinase phosphatase 1, MKP-1, Protein-tyrosine phosphatase CL100, DUSP1, CL100, MKP1, PTPN10, VH1
Protein Accession Number:
P28562
Protein Gi Number:
1346900
Ncbi Gene Id Number:
1843
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein hasintrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.
Background Reference 1:
Wu,W., et al., J. Biol. Chem. 280 (6), 4117-4124 (2005).
Background Reference 2:
Small,G.W., et al., Mol. Pharmacol. 66 (6), 1478-1490 (2004).
Background Reference 3:
Chandrasekharan,U.M., et al., J. Biol. Chem. 279 (45), 46678-46685 (2004).
Background Reference 4:
Mizuno,R., et al., J. Urol. 172 (2), 723-727 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-488

List Size:
400 ul

List Price:

$469.00

Out of stock

Datasheet
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