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Western blot analysis of DUSP2 Antibody in mouse liver tissue lysates (35ug/lane)
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DUSP2 Antibody in mouse liver tissue lysates (35ug/lane)

DUSP2 Antibody

CATALOG NUMBER: 63-775

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This DUSP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-290 amino acids from the C-terminal region of human DUSP2.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DUSP2
Additional Names:
Dual specificity protein phosphatase 2, Dual specificity protein phosphatase PAC-1, DUSP2, PAC1
Protein Accession Number:
Q05923
Protein Gi Number:
464334
Ncbi Gene Id Number:
1844
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DUSP2 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli.
Background Reference 1:
Caunt,C.J., et.al., J. Biol. Chem. 283 (10), 6241-6252 (2008)
Background Reference 2:
Baranyai,R., et.al., Neuropsychobiology 57 (3), 146-150 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-775

List Size:
400 ul

List Price:

$495.00

Datasheet
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