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Western blot analysis of DUSP3 using rabbit polyclonal DUSP3 Antibody (C171) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DUSP3 gene.
DUSP3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DUSP3 using rabbit polyclonal DUSP3 Antibody (C171) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DUSP3 gene.

DUSP3 Antibody

CATALOG NUMBER: 63-474

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DUSP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-185 amino acids from the C-terminal region of human DUSP3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
20 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DUSP3
Additional Names:
Dual specificity protein phosphatase 3, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase, VHR, DUSP3, VHR
Protein Accession Number:
P51452
Protein Gi Number:
1718191
Ncbi Gene Id Number:
1845
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. The gene for this protein maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer.
Background Reference 1:
Alonso, A., et al., Nat. Immunol. 4(1):44-48 (2003).
Background Reference 2:
Alonso, A., et al., J. Biol. Chem. 276(7):4766-4771 (2001).
Background Reference 3:
Todd, J.L., et al., J. Biol. Chem. 274(19):13271-13280 (1999).
Background Reference 4:
Kamb, A., et al., Genomics 23(1):163-167 (1994).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-474

List Size:
400 ul

List Price:

$495.00

Datasheet
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