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Western blot analysis of anti-DUSP4 Antibody in HepG2 cell line lysates (35ug/lane)
Western blot analysis of anti-DUSP4 Pab in mouse brain tissue lysate (35ug/lane).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-DUSP4 Antibody in HepG2 cell line lysates (35ug/lane)

DUSP4 Antibody

CATALOG NUMBER: 63-475

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This DUSP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the C-terminal region of human DUSP4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DUSP4
Additional Names:
Dual specificity protein phosphatase 4, Dual specificity protein phosphatase hVH2, Mitogen-activated protein kinase phosphatase 2, MAP kinase phosphatase 2, MKP-2, DUSP4, MKP2, VH2
Protein Accession Number:
Q13115
Protein Gi Number:
2499745
Ncbi Gene Id Number:
1846
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DUSP4 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP4 inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus.
Background Reference 1:
Chen, P., et al., J. Biol. Chem. 276(31):29440-29449 (2001).
Background Reference 2:
Smith, A., et al., Genomics 42(3):524-527 (1997).
Background Reference 3:
Chu, Y., et al., J. Biol. Chem. 271(11):6497-6501 (1996).
Background Reference 4:
King, A.G., et al., Oncogene 11(12):2553-2563 (1995).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-475

List Size:
400 ul

List Price:

$495.00

Datasheet
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