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43-212 (0.3ug/ml) staining of Human (A), (Mouse) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-212 (0.3ug/ml) staining of Pig Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-212 (0.3ug/ml) staining of Human (A), (Mouse) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DUSP6 / MKP3 Antibody

CATALOG NUMBER: 43-212

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-PSNQNVYQVDSLQST
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Mouse and Rat Heart lysates and 40kDa plus 26kDa bands observed in Human and Pig Heart lysates (calculated MW of 42.3kDa according to NP_001937.2 and 26.5kDa according to NP_073143.2). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001937.2; NP_073143.2).
Predicted Molecular Weight:
Approx 26kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DUSP6 / MKP3
Additional Names:
DUSP6, dual specificity phosphatase 6, MKP3, PYST1, MAP kinase phosphatase 3, dual specificity protein phosphatase 6, dual specificity protein phosphatase PYST1, mitogen-activated protein kinase phosphatase 3, serine/threonine specific protein phosphatase
Protein Accession Number:
NP_001937.2,NP_073143.2
Protein Gi Number:
108860971
Ncbi Gene Id Number:
1848
Background Reference 1:
Geetha N, Mihaly J, Stockenhuber A, Blasi F, Uhrin P, Binder BR, Freissmuth M, Breuss JM.Signal integration and coincidence detection in the mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) cascade: concomitant activation of receptor tyrosine kinases and of LRP-1 leads to sustained ERK phosphorylation via down-regu

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-212

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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