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Western blot analysis of DUSP7 using DUSP7 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DUSP7 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of DUSP7 using DUSP7 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DUSP7 gene (Lane 2).

DUSP7 Antibody

CATALOG NUMBER: 63-477

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This DUSP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-127 amino acids from the N-terminal region of human DUSP7.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DUSP7
Additional Names:
Dual specificity protein phosphatase 7, Dual specificity protein phosphatase PYST2, DUSP7, PYST2
Protein Accession Number:
Q16829
Protein Gi Number:
338817906
Ncbi Gene Id Number:
1849
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DUSP7 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP)kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associatedwith cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli.
Background Reference 1:
Immunol. Lett. 92 (1-2), 149-156 (2004)
Background Reference 2:
Oncogene 22 (48), 7649-7660 (2003)
Background Reference 3:
Meth. Enzymol. 366, 103-113 (2003)
Background Reference 4:
J. Cell. Sci. 111 (PT 22), 3389-3399 (1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-477

List Size:
400 ul

List Price:

$495.00

Datasheet
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