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45-507 (2ug/ml) staining of Human Cerebellum (A), Mouse (B), and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-507 (2ug/ml) staining of Human Cerebellum (A), Mouse (B), and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DUSP8 Antibody

CATALOG NUMBER: 45-507

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: AGDRLPRKVMDAK-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55-58kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 65.8kDa according to Human NP_004411.2, 68.8kDa according to Mouse NP_032774.1 and 66.8kDa according to Rat NP_001101980.1 ). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is also expected to recognise the hypothetical human protein similar to dual specificity phosphatase 8 (XM_114902), which is virtually identical.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DUSP8
Additional Names:
DUSP8, dual specificity phosphatase 8, HB5, HVH8, HVH-5, H1 phosphatase, vaccinia virus homolog, serine/threonine specific protein phosphatase, C11orf81, FLJ42476, FLJ42958, OTTHUMP00000164487
Protein Accession Number:
NP_004411.2
Protein Gi Number:
4758212
Ncbi Gene Id Number:
1850
Background Reference 1:
Martell KJ, Seasholtz AF, Kwak SP, Clemens KK, Dixon JE. hVH-5: a protein tyrosine phosphatase abundant in brain that inactivates mitogen-activated protein kinase. J Neurochem. 1995 Oct;65(4):1823-33.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-507

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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