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Western blot analysis of anti-DUSP8 Pabin Hela cell line lysate (35ug/lane).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-DUSP8 Pabin Hela cell line lysate (35ug/lane).

DUSP8 Antibody

CATALOG NUMBER: 63-478

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DUSP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-487 amino acids from the C-terminal region of human DUSP8.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DUSP8
Additional Names:
Dual specificity protein phosphatase 8, Dual specificity protein phosphatase hVH-5, DUSP8, C11orf81, VH5
Protein Accession Number:
Q13202
Protein Gi Number:
223590200
Ncbi Gene Id Number:
1850
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DUSP8 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP8 inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin.
Background Reference 1:
Berger, I.R., et al., Cancer Genet. Cytogenet. 159(2):155-159 (2005).
Background Reference 2:
Hink, R.L., et al., Genomics 8(3):305-312 (2003).
Background Reference 3:
Nesbit, M.A., et al., Genomics 42(2):284-294 (1997).
Background Reference 4:
Martell, K.J., et al., J. Neurochem. 65(4):1823-1833 (1995).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-478

List Size:
400 ul

List Price:

$495.00

Datasheet
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