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Western Blot at 1:1000 dilution + SW620 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of lysate from A431 cell line, using DUT Antibody at 1:1000 at each lane.
DUT Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + SW620 whole cell lysates Lysates/proteins at 20 ug per lane.

DUT Antibody

CATALOG NUMBER: 57-557

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This DUT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the C-terminal region of human DUT.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
27 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DUT
Additional Names:
Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, dUTPase, dUTP pyrophosphatase, DUT
Protein Accession Number:
P33316
Protein Gi Number:
347595814
Ncbi Gene Id Number:
1854
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Background Reference 1:
Takatori, H., et al. Liver Int. 30(3):438-446(2010) Quesada-Soriano, I., et al. Biochimie 92(2):178-186(2010) Chanson, A., et al. Am. J. Clin. Nutr. 89(6):1927-1936(2009) Takacs, E., et al. FEBS Lett. 583(5):865-871(2009) Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-557

List Size:
400 ul

List Price:

$495.00

Datasheet
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