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43-264 (1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-264 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-264 (1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Dvl1 Antibody

CATALOG NUMBER: 43-264

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-PQLEEAPLTVKSD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80kDa band observed in lysates of cell line NIH3T3 (calculated MW of 75.4kDa according to NP_034221.3). An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 80kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Dvl1
Additional Names:
Dvl1, dishevelled, dsh homolog 1 (Drosophila), RP23-242K15.15-003, Dvl, mKIAA4029, DSH homolog 1, dishevelled-1, segment polarity protein dishevelled homolog DVL-1
Protein Accession Number:
NP_034221.3,NP_001343310.1
Protein Gi Number:
47933344
Ncbi Gene Id Number:
13542
Background Reference 1:
Huang T, Xie Z, Wang J, Li M, Jing N, Li L.Nuclear factor of activated T cells (NFAT) proteins repress canonical Wnt signaling via its interaction with Dishevelled (Dvl) protein and participate in regulating neural progenitor cell proliferation and differentiation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-264

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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