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Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: NIH/3T3 whole cell lysates Lane 3: K562 whole cell lysates Lysates/proteins at 20 ug per lane.
DVL3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of DVL3 Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: NIH/3T3 whole cell lysates Lane 3: K562 whole cell lysates Lysates/proteins at 20 ug per lane.

DVL3 Antibody

CATALOG NUMBER: 56-188

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This DVL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 530-557 amino acids from the C-terminal region of human DVL3.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
78 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DVL3
Additional Names:
Segment polarity protein dishevelled homolog DVL-3, Dishevelled-3, DSH homolog 3, DVL3, KIAA0208
Protein Accession Number:
Q92997
Protein Gi Number:
6919875
Ncbi Gene Id Number:
1857
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of a multi-gene family which sharesstrong similarity with the Drosophila dishevelled gene, dsh. TheDrosophila dishevelled gene encodes a cytoplasmic phosphoproteinthat regulates cell proliferation.
Background Reference 1:
Gnad, T., et al. Mol. Cancer 9, 31 (2010) :
Background Reference 2:
Bouteille, N., et al. Oncogene 28(28):2569-2580(2009)
Background Reference 3:
Fukukawa, C., et al. Oncogene 28(8):1110-1120(2009)
Background Reference 4:
Wei, Q., et al. Lung Cancer 62(2):181-192(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-188

List Size:
400 ul

List Price:

$495.00

Datasheet
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