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Lane A - 42-509 (1ug/ml) staining of HEK293 overexpressing Human DYDC1 lysate (10ug protein in RIPA buffer) Lane B - 42-509 (0.1ug/ml) staining of HEK293 mock-transfected lysate (10ug protein in RIPA buffer). Lane C - anti-MYC Tag (1/1000) stain
42-509 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Lane A - 42-509 (1ug/ml) staining of HEK293 overexpressing Human DYDC1 lysate (10ug protein in RIPA buffer) Lane B - 42-509 (0.1ug/ml) staining of HEK293 mock-transfected lysate (10ug protein in RIPA buffer). Lane C - anti-MYC Tag (1/1000) stain

DYDC1 Antibody

CATALOG NUMBER: 42-509

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-TLAEISDRYGAPN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:n transfected HEK293 transiently expressing Human DYDC1 (myc and DYKDDDDK tagged), a band of approx. 28kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. This 28kDa band is also observed in Human, Mouse and Rat Testis lysates. Recommended concentration: 0.1-1ug/ml.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DYDC1
Additional Names:
DYDC1, DPY30 domain containing 1, DPY30D1, FLJ43920, bA36D19.5, BC019250 protein, OTTHUMP00000019963
Protein Accession Number:
NP_620167.1
Protein Gi Number:
20270377
Ncbi Gene Id Number:
143241

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-509

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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