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Western blot analysis using Dynamin1 monoclonal antibody against truncated Dynamin1 recombinant protein SKN - SH cell lysate and NIH / 3T3 cell lysate .
Immunohistochemical analysis of paraffin - embedded human cerebellum tissue showing membrane location using Dynamin1 antibody with DAB staining.
Western blot analysis using Dynamin1 monoclonal antibody against truncated Dynamin1 recombinant protein SKN - SH cell lysate and NIH / 3T3 cell lysate .

Dynamin-1 Antibody [3G4B6]

CATALOG NUMBER: 32-146

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant Dynamin-1 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG2a
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Dynamin-1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DNM1
Additional Names:
DNMDNM
Protein Accession Number:
Q05193
Protein Gi Number:
172046078
Ncbi Gene Id Number:
1759
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Dynamin-1 (Dyn1), with 864-amino acid protein (about 95 kDa), belongs to the dynamin family. Dynamin-1 (neuron-specific), dynamin-2 (ubiquitously expressed), and dynamin-3 (expressed only in the testis, brain, and lung), constitute the dynamin family. Members of the dynamin family are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin-1 is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C. Dynamin-1 is a large GTPase enzyme required in membrane constriction and fission during multiple forms of endocytosis. Dynamin-1 is also a key molecule required for the recycling of synaptic vesicles in neurons, and it has been known that dynamin-1 gene expression is induced during neuronal differentiation.
Background Reference 1:
Annie Quan and Phillip J. Robinson. Methods Enzymol. 2005; 404:556-69.
Background Reference 2:
Jiyun Yoo, Moon-Jin Jeong, Byoung-Mog Kwon. J. Biol. Chem., Mar 2002; 277: 11904 – 11909.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-146

List Size:
0.1 mL

List Price:

$515.00

Datasheet
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