Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot of a forskolin stimulated rat hippocampal lysate showing phospho-specific immunolabeling of the ~95k Dynamin protein phosphorylated at Ser774.
Western blot of a forskolin stimulated rat hippocampal lysate showing phospho-specific immunolabeling of the ~95k Dynamin protein phosphorylated at Ser774.

Dynamin (phospho Ser774) Antibody

CATALOG NUMBER: XPS-1006

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Sheep
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Sheep
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Dynamin (Ser774) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser774 of human, mouse and rat dynamin.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Dynamin antibody for phospho-Ser774 dynamin labels the purified protein phosphorylated in vitro by Cdk5 but not PKC (Tan, et al. 2003). The labeling of the dynamin band in Western blots of rat brain was blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Sheep anti- Dynamin (Ser774) does not cross react with other purified substrates of CDK5 (e.g. amphiphysin and synapsin). Applications include Immunoblotting (IB), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blots (WB). Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IB and WB.
Predicted Molecular Weight:
95
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
Ncbi Official Symbol:
Dnm1
Protein Accession Number:
P21575
Protein Gi Number:
190358918
Ncbi Gene Id Number:
140694
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
Background Reference 1:
Tan, T.C., Valova, V.A., Malladi, C.S., Graham, M.E., Berven, L.A., Jupp, O.J., Hansra, G, McClure, S.J., Surcevic, B, Boadle, R.A., Larsen, M.R., Cousin, M.A., Robinson, P.J., "Cdk5 is essential for synaptic vesicle endocyotosis," Nat. Cell Biol., 8 (2003) 701-710.
Background Reference 2:
Graham, M.E., O'Callaghan, D.W., McMahon, H.T., and Burgoyne, R.D., "Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size," Proc. Natl. Acad. Sci. USA, 99 (2002) 7124 - 7129.
Background Reference 3:
Tsuboi, T., Terakawa, S., Scalettar, B.A., Fantus, C., Roder, J., and Jeromin, A., "Sweeping model of dynamin activity - Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins," J. Biol. Chem., 277 (2002) 15,957-15,961.
Background Reference 4:
Cousin, M.A., Tan, T.C., and Robinson, P.J., "Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin," J. Neurochem., 76 (2001) 105-116.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1006

List Size:
0.1 mL

List Price:

$540.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369