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43-184 (0.5ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-184 (0.5ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

DYRK1A Antibody

CATALOG NUMBER: 43-184

Clonality:
Polyclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: PHSHQYSDRRQPN-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 80kDa band observed in lysates of cell line K562 (calculated MW of 85.6kDa according to NP_001387.2). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour.Immunoprecipitation: This product has been successfully used in IP on Human: Widowati et al, BMC Res Notes. 2018 May 15;11(1):297. PMID: 29764512.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_001387.2; NP_569120.1; NP_567824.1; NP_569122.1). This antibody is not expected to cross-react with DYRK1B.
Predicted Molecular Weight:
Approx 80kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DYRK1A
Additional Names:
DYRK1A, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, DYRK, DYRK1, HP86, MNB, MNBH, MRD7, MNB/DYRK protein kinase, OTTHUMP00000109090, OTTHUMP00000109091, OTTHUMP00000109094, OTTHUMP00000174799, dual specificity YAK1-related kinase, dual specificity tyrosine-phosphorylation-regulated kinase 1A, hMNB, mnb protein kinase homolog hp86, protein kinase minibrain homolog, serine/threonine kinase MNB, serine/threonine-specific protein kinase
Protein Accession Number:
NP_001387.2,NP_569120.1,NP_567824.1,NP_569122.1
Protein Gi Number:
18765758
Ncbi Gene Id Number:
1859
Background Reference 1:
Miyata Y, Nishida E.DYRK1A binds to an evolutionarily conserved WD40-repeat protein WDR68 and induces its nuclear translocation.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-184

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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