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45-510 staining (0.5ug/ml) of Human Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
45-510 staining (0.5ug/ml) of Human Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Dysadherin Antibody

CATALOG NUMBER: 45-510

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: GKCRQLSRLCRNHCR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 38kDa band observed in Human Spleen lysates (calculated MW of 19.5 kDa according to NP_054883 and NP_659003). The band was successfully blocked by incubation with the immunizing peptide. According to the literature this protein is O-glycosylated (PMID: 12672699), which may explain the size difference observed.
Specificity:
NP_054883.3, NP_659003.1and NP_001158077.1 are variants that represent the same protein.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FXYD5
Additional Names:
FXYD5, dysadherin, FXYD domain containing ion transport regulator 5, RIC, IWU1, IWU-1, dysad, HSPC113, KCT1, OIT2, PRO6241, FXYD domain-containing ion transport regulator 5, keratinocytes associated transmembrane protein 1, DYSAD, HSPC113
Protein Accession Number:
NP_054883.3,NP_659003.1,NP_001158077.1
Protein Gi Number:
7661784
Ncbi Gene Id Number:
53827
Background Reference 1:
Ino Y, Gotoh M, Sakamoto M, Tsukagoshi K, Hirohashi S. Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis. Proc Natl Acad Sci USA. 2002 Jan 8;99(1):365-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-510

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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