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Western blot analysis of extracts of 293T cells, using DZIP3 Antibody (22-760) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Western blot analysis of extracts of 293T cells, using DZIP3 Antibody (22-760) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

DZIP3 Antibody

CATALOG NUMBER: 22-760

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human DZIP3 (NP_055463.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
293T
Predicted Molecular Weight:
Observed: 138kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DZIP3
Additional Names:
PPP1R66, UURF2, hRUL138, E3 ubiquitin-protein ligase DZIP3, DAZ interacting protein 3, zinc finger, DAZ-interacting protein 3, RING-type E3 ubiquitin transferase DZIP3, RNA-binding RING-H2 protein-ubiquitin ligase, RNA-binding ubiquitin ligase of 138 kDa, UURF2 ubiquitin ligase, human RNA-binding ubiquitin ligase of 138 kDa, protein phosphatase 1, regulatory subunit 66, zinc finger DAZ interacting protein 3
Ncbi Gene Id Number:
9666
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-760

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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