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IHC testing of FFPE human skin with E-Cadherin antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
IHC testing of FFPE human placenta with E-Cadherin antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
Western blot testing of 1) partial recombinant protein and 2) human stomach lysate using E-Cadherin antibody at 0.5ug/ml. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
IHC testing of FFPE human skin with E-Cadherin antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.

E-Cadherin Antibody [ECD1-2]

CATALOG NUMBER: 34-050

Clonality:
Monoclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT
Immunofluorescence: 0.5-1 ug/ml
Optimal dilution of the E-Cadherin antibody should be determined by the researcher.
Specificity:
Not suitable for Mouse, Rat sample testing
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CDH1
Additional Names:
Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO
Ncbi Gene Id Number:
999
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-050

List Size:
100 ug

List Price:

$626.00

Datasheet
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