E. coli O157 Antibody [9.88]

CATALOG NUMBER: 33-674

Clonality:
Monoclonal
Tested Applications:
ELISA, IF
Host Species:
Mouse
Species Reactivity:
E. coli
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
E. coli
Immunogen:
A crude sonicate of E. coli O157 was used as the immunogen for the E. coli O157 antibody.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF
Application Note:
ELISA: 1-5 ug/ml; order BSA free formulation for coating
IF: 1-2 ug/ml
Optimal dilution of the E. coli O157 antibody should be determined by the researcher.
Purification:
PEG precipitation followed by dialysis in 1X PBS
Clonality:
Monoclonal
Isotype:
IgM, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-674

List Size:
100 ug

List Price:

$626.00

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