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Antibody used in WB on Human Daudi cells at 0.2-1 ug/ml.
Antibody used in WB on Human Daudi cells at 0.2-1 ug/ml.

E2F1 Antibody

CATALOG NUMBER: 31-046

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
E2F1 antibody can be used for detection of E2F1 by ELISA at 1:312500. E2F1 antibody can be used for detection of E2F1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Predicted Molecular Weight:
47 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store E2F1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
E2F1
Additional Names:
E2F1, RBP3, E2F-1, RBAP1, RBBP3
Protein Accession Number:
NP_005216
Protein Gi Number:
12669911
Ncbi Gene Id Number:
1869
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Background Reference 1:
Unoki, M., et al., (2004) Oncogene 23 (46), 7601-7610.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-046

List Size:
100 ul

List Price:

$519.00

Datasheet
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