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Western Blot at 1:1000 dilution Lane 1: NIH-3T3 whole cell lysates Lane 2: A431 whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + Hela whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of E2F1 Antibody (H357) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293T cell line lysate.
Western Blot at 1:1000 dilution Lane 1: NIH-3T3 whole cell lysates Lane 2: A431 whole cell lysates Lysates/proteins at 20 ug per lane.

E2F1 Antibody

CATALOG NUMBER: 63-021

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from human E2F1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
E2F1
Additional Names:
Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1, E2F1, RBBP3
Protein Accession Number:
Q01094
Protein Gi Number:
400928
Ncbi Gene Id Number:
1869
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another two members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Background Reference 1:
O'Donnell, K.A., et al., Nature 435(7043):839-843 (2005). Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005). Joshi, B., et al., Oncogene 24(13):2204-2217 (2005). Saberwal, G., et al., Int. J. Hematol. 80(2):146-154 (2004). Chaussepied, M., et al., Mol. Cell 16(5):831-837 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-021

List Size:
400 ul

List Price:

$495.00

Datasheet
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