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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse kidney tissue lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 ug per lane.

E2F2 Antibody

CATALOG NUMBER: 55-208

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This E2F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the Central region of human E2F2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
E2F2
Additional Names:
Transcription factor E2F2, E2F-2, E2F2
Protein Accession Number:
Q14209
Protein Gi Number:
2494228
Ncbi Gene Id Number:
1870
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
E2F2 is a member of the E2Ffamily of transcription factors. The E2F family plays a crucialrole in the control of cell cycle and action of tumor suppressorproteins and is also a target of the transforming proteins of smallDNA tumor viruses. The E2F proteins contain several evolutionallyconserved domains found in most members of the family. Thesedomains include a DNA binding domain, a dimerization domain whichdetermines interaction with the differentiation regulatedtranscription factor proteins (DP), a transactivation domainenriched in acidic amino acids, and a tumor suppressor proteinassociation domain which is embedded within the transactivationdomain. This protein and another 2 members, E2F1 and E2F3, have anadditional cyclin binding domain. This protein binds specificallyto retinoblastoma protein pRB in a cell-cycle dependent manner, andit exhibits overall 46% amino acid identity to E2F1. [provided byRefSeq].
Background Reference 1:
Revenko, A.S., et al. Mol. Cell. Biol. 30(22):5260-5272(2010)
Background Reference 2:
Hayami, S., et al. Mol. Cancer 9, 59 (2010) :
Background Reference 3:
Chen, J., et al. Cancer Causes Control 20(9):1769-1777(2009)
Background Reference 4:
Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-208

List Size:
400 ul

List Price:

$495.00

Datasheet
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