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Antibody used in WB on Human Thymus at 0.2-1 ug/ml.
Antibody used in WB on Human Thymus at 0.2-1 ug/ml.

E2F3 Antibody

CATALOG NUMBER: 28-786

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
E2F3 antibody can be used for detection of E2F3 by ELISA at 1:62500. E2F3 antibody can be used for detection of E2F3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate
Predicted Molecular Weight:
37 kDa, 49 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store E2F3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
E2F3
Additional Names:
E2F3, E2F-3
Protein Accession Number:
CAH18140
Protein Gi Number:
51476300
Ncbi Gene Id Number:
1871
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. E2F3 binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
Background Reference 1:
Koehrer, K., et al., (2004) The German cDNA Consortium.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-786

List Size:
100 ul

List Price:

$519.00

Datasheet
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