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43-404 (1ug/ml) staining of Mouse fetal Lung lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
43-404 (1ug/ml) staining of Mouse fetal Lung lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

E2F3 Antibody

CATALOG NUMBER: 43-404

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: DLEKLPLVEDFMCS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 60kDa and 48kDa band observed in Mouse fetal Lung lysates (calculated MW of 48.8kDa according to NP_034223.1). The 60kDa band was also observed in nuclear lysates of cell line NIH3T3 and it corresponds with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.
Predicted Molecular Weight:
Approx 60kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
E2F3
Additional Names:
E2f3, E2F transcription factor 3, E2F3b, E2f3a, mKIAA0075, OTTMUSP00000000663
Protein Accession Number:
NP_034223.1
Protein Gi Number:
51476300
Ncbi Gene Id Number:
1871
Background Reference 1:
Wu L, Timmers C, Maiti B, Saavedra HI, Sang L, Chong GT, Nuckolls F, Giangrande P, Wright FA, Field SJ, Greenberg ME, Orkin S, Nevins JR, Robinson ML, Leone G, The E2F1-3 transcription factors are essential for cellular proliferation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-404

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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