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Western blot analysis of E2F3 in human lymph node tissue lysate with E2F3 antibody at 1 μg/ml.
Immunohistochemistry of E2F3 in human lymph node tissue with E2F3 antibody at 5 μg/ml.
Immunofluorescence of E2F3 in human lymph node tissue with E2F3 antibody at 20 μg/ml.
Western blot analysis of E2F3 in human lymph node tissue lysate with E2F3 antibody at 1 μg/ml.
Trial Size

E2F3 Antibody

CATALOG NUMBER: 7929

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3.
The immunogen is located within amino acids 150 - 200 of E2F3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
E2F3 antibody can be used for detection of E2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
E2F3 antibody is human and mouse reactive. At least three isoforms of E2F3 are known to exist; this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.
Positive Control 1:
Cat. No. 1369 - Human Lymph node Tissue Lysate
Positive Control 2:
Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
Predicted Molecular Weight:
Predicted: 51 kDa
Observed: 56 kDa
Purification:
E2F3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
E2F3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
E2F3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
E2F3
Additional Names:
E2F transcription factor 3, E2F-3
Protein Accession Number:
NP_001940
Protein Gi Number:
4503433
Ncbi Gene Id Number:
1871
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The E2F transcription factor 3 (E2F3) is a member of a small family of transcription factors that function through binding of DP interaction partner proteins. E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle (1). Like the related E2F1 and E2F2, E2F3 is essential for cellular proliferation and progression through the cell cycle (2). Altered copy number and activity of this gene have been observed in a number of human cancers (3).
Background Reference 1:
Lees JA, Saito M, Vidal M, et al. The retinoblastoma protein binds to a family of E2F transcription factors. Mol. Cell. Biol. 1993; 13:7813-25.
Background Reference 2:
Wu L, Timmers C, Maiti B, et al. The E2F1-3 transcription factors are essential for cellular proliferation. Nature 2001; 414:457-62.
Background Reference 3:
DeGregori J. The genetics of the E2F family of transcription factors: shared functions and unique roles. Biochim. Biophys. Acta. 2002; 1602:131-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7929

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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