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Western blot analysis of extracts of MCF-7 cells, using E2F6 antibody (18-784) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophageal cancer using E2F6 antibody (18-784) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using E2F6 antibody (18-784) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using E2F6 antibody (18-784). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 3ug E2F6 antibody (18-784). Western blot was performed from the immunoprecipitate using E2F6 antibody (18-784) at a dilition of 1:500.
Western blot analysis of extracts of MCF-7 cells, using E2F6 antibody (18-784) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

E2F6 Antibody

CATALOG NUMBER: 18-784

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
MCF7
Predicted Molecular Weight:
Observed: 38kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
E2F6
Additional Names:
E2F6, E2F-6
Ncbi Gene Id Number:
1876
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-784

List Size:
100 uL

List Price:

$436.00

Datasheet
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