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Western blot analysis in A549 cell line lysates (35ug/lane).
EAPII Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of EAPII Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549 cell line lysates (35ug/lane).

EAPII Antibody

CATALOG NUMBER: 55-723

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EAPII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-272 amino acids from the C-terminal region of human EAPII.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
41 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TDP2
Additional Names:
Tyrosyl-DNA phosphodiesterase 2, Tyr-DNA phosphodiesterase 2, hTDP2, 314-, 5'-tyrosyl-DNA phosphodiesterase, 5'-Tyr-DNA phosphodiesterase, ETS1-associated protein 2, ETS1-associated protein II, EAPII, TRAF and TNF receptor-associated protein, Tyrosyl-RNA phosphodiesterase, VPg unlinkase, TDP2, EAP2, TTRAP
Protein Accession Number:
O95551
Ncbi Gene Id Number:
51567
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of a superfamily of divalentcation-dependent phosphodiesterases. The encoded protein associateswith CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptorassociated factors (TRAFs), and inhibits nuclear factor-kappa-Bactivation. This protein has sequence and structural similaritieswith APE1 endonuclease, which is involved in both DNA repair andthe activation of transcription factors.
Background Reference 1:
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Background Reference 2:
Wang, B.Y., et al. Mol. Biol. Rep. 37(6):2809-2816(2010)
Background Reference 3:
Iijima, M., et al. Neurology 73(17):1348-1352(2009)
Background Reference 4:
Cortes Ledesma, F., et al. Nature 461(7264):674-678(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-723

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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