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Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane)

EARS2 Antibody

CATALOG NUMBER: 63-004

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-523 amino acids from the C-terminal region of human EARS2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EARS2
Additional Names:
Probable glutamate--tRNA ligase, mitochondrial, Glutamyl-tRNA synthetase, GluRS, EARS2, KIAA1970
Protein Accession Number:
Q5JPH6
Protein Gi Number:
117949790
Ncbi Gene Id Number:
124454
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Glutamyl-tRNA synthetase (GluRS or EARS2) a class I aminoacyl-tRNA synthetase (aaRS), is primarily responsible for the glutamylation of tRNAGlu. It is part of the ?minimal set?of seventeen aaRSs found in every living organism and its presence is essential for the viability of cells.
Background Reference 1:
Bonnefond,L.,Biochemistry 44 (12), 4805-4816 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-004

List Size:
400 ul

List Price:

$495.00

Datasheet
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