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Western blot analysis of ECE-1 Antibody in A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skin tissue reacted with ECE-1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ECE-1 Antibody in A2058 cell line lysates (35ug/lane)

ECE-1 Antibody

CATALOG NUMBER: 62-503

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This ECE-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 528-556 amino acids from the C-terminal region of human ECE-1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
87 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ECE1
Additional Names:
Endothelin-converting enzyme 1, ECE-1, ECE1
Protein Accession Number:
P42892
Protein Gi Number:
1706563
Ncbi Gene Id Number:
1889
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ECE-1 is involved in proteolytic processing of endothelin precursors to biologically active peptides.
Background Reference 1:
Cottrell,G.S., et.al., J. Biol. Chem. 284 (33), 22411-22425 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-503

List Size:
400 ul

List Price:

$495.00

Datasheet
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