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Immunohistochemical analysis of paraffin-embedded H. prostate section using ECE2 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis in U251 cell line lysates (35ug/lane).
Immunohistochemical analysis of paraffin-embedded H. prostate section using ECE2 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

ECE2 Antibody

CATALOG NUMBER: 60-991

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This ECE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 551-579 amino acids from the Central region of human ECE2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
100 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ECE2
Additional Names:
Endothelin-converting enzyme 2, ECE-2, Methyltransferase-like region, 211-, Endothelin-converting enzyme 2 region, ECE2, KIAA0604
Protein Accession Number:
O60344
Ncbi Gene Id Number:
9718
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta-amyloid peptide accumulation in brain. May also have methyltransferase activity.
Background Reference 1:
Lorenzo M.-N., et al. Biochim. Biophys. Acta 1522:46-52(2001).
Background Reference 2:
Funke-Kaiser H., et al. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
Background Reference 3:
Nagase T., et al. DNA Res. 5:31-39(1998).
Background Reference 4:
Clark H.F., et al. Genome Res. 13:2265-2270(2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-991

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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