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Western blot analysis of extracts of various cell lines, using ECH1 Antibody (14-714) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human tonsil using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Western blot analysis of extracts of various cell lines, using ECH1 Antibody (14-714) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

ECH1 Antibody

CATALOG NUMBER: 14-714

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human ECH1 (NP_001389.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
A375
Positive Control 2:
BxPC-3
Positive Control 3:
HepG2
Positive Control 4:
Jurkat
Positive Control 5:
MCF7
Positive Control 6:
K-562
Predicted Molecular Weight:
Observed: 36kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ECH1
Additional Names:
ECH1, HPXEL
Ncbi Gene Id Number:
1891
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-714

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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