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Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis in A549 cell line lysates (35ug/lane).

ECH1 Antibody

CATALOG NUMBER: 59-987

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ECH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-250 amino acids from the C-terminal region of human ECH1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ECH1
Additional Names:
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, 533-, ECH1
Protein Accession Number:
Q13011
Protein Gi Number:
82654933
Ncbi Gene Id Number:
1891
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the hydratase/isomerasesuperfamily. The gene product shows high sequence similarity toenoyl-coenzyme A (CoA) hydratases of several species, particularlywithin a conserved domain characteristic of these proteins. Theencoded protein, which contains a C-terminal peroxisomal targetingsequence, localizes to the peroxisome. The rat ortholog, whichlocalizes to the matrix of both the peroxisome and mitochondria,can isomerize 3-trans,5-cis-dienoyl-CoA to2-trans,4-trans-dienoyl-CoA, indicating that it is adelta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions inthe auxiliary step of the fatty acid beta-oxidation pathway.Expression of the rat gene is induced by peroxisome proliferators.
Background Reference 1:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 2:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 3:
Kovalyov, L.I., et al. Biochemistry Mosc. 71(4):448-453(2006)
Background Reference 4:
Goehler, H., et al. Mol. Cell 15(6):853-865(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-987

List Size:
400 ul

List Price:

$495.00

Datasheet
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