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Western blot analysis of ECRG2 in A-20 cell lysate with ECRG2 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Immunohistochemistry of ECRG2 in rat lung tissue with ECRG2 antibody at 5 μg/mL.
Western blot analysis of ECRG2 in A-20 cell lysate with ECRG2 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Trial Size

ECRG2 Antibody

CATALOG NUMBER: 6523

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ECRG2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human ECRG2.
The immunogen is located within the last 50 amino acids of ECRG2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
ECRG2 antibody can be used for detection of ECRG2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Specificity:
ECRG2 antibody is predicted to not cross-react with other ECRG family members
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Predicted Molecular Weight:
Predicted: 9 kDa
Observed: 14 kDa
Purification:
ECRG2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
ECRG2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ECRG2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPINK7
Additional Names:
ECRG2 Antibody: ECG2, ECRG2, ECG2, UNQ745/PRO1474, Serine protease inhibitor Kazal-type 7, Esophagus cancer-related gene 2 protein, ECRG-2
Protein Accession Number:
AAI09386
Protein Gi Number:
14211875
Ncbi Gene Id Number:
84651
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ECRG2 Antibody: The esophageal cancer-susceptibility gene 2 (ECRG2), also known as SPINK7, is a novel tumor suppressor gene identified from the human esophagus. It interacts directly with metallothionein 2A and urokinase-type plasminogen activator (uPA), and downregulates the activity of uPA, leading to reduced cancer cell migration, invasion and metastasis. ECRG2 forms a complex with uPA and its receptor uPAR, modifying the dynamic association of uPAR with beta1 integrins and disrupting the Src/MAP kinase pathway that normally stimulates cell migration and invasion. ECRG2 may thus represent a novel therapeutic target for cancer.
Background Reference 1:
Cui Y, Wang J, Zhang X, et al. ECRG2, a novel candidate of tumor suppressor gene in the esophageal carcinoma, interacts directly with metallothionein 2A and links to apoptosis. Biochem. Biophys. Res. Commun. 2003; 302:904-15.
Background Reference 2:
Huang G, Hu Z, Li M, et al. ECRG2 inhibits cancer cell migration, invasion and metastasis through the down-regulation of uPA/plasmin activity. Carcinogenesis 2007; 28:2274-81
Background Reference 3:
Cheng X, Shen Z, Yin L, et al. ECRG2 regulates cell migration/invasion through the urokinase-type plasmin activator receptor (uPAR)/beta1 integrin pathway. J. Biol. Chem. 2009; 284:30897-906

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6523

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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