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Western blot analysis of ECSIT in human heart cell lysates with ECSIT antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunohistochemistry of ECSIT in mouse heart cells with ECSIT antibody at 2 μg/mL.
Western blot analysis of ECSIT in human heart cell lysates with ECSIT antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
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ECSIT Antibody

CATALOG NUMBER: 3377

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
ECSIT antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human ECSIT.
The immunogen is located within amino acids 290 - 340 of ECSIT.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
ECSIT antibody can be used for detection of ECSIT by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
ECSIT Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
ECSIT Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ECSIT antibody can be stored at 4°C, stable up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ECSIT
Additional Names:
ECSIT Antibody: SITPEC, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, Protein SITPEC
Protein Accession Number:
NP_057665
Protein Gi Number:
20149633
Ncbi Gene Id Number:
51295
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ECSIT Antibody: Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.
Background Reference 1:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 2:
Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
Background Reference 3:
Janssens S and Beyaert R. Functional diversity and regulation of different interleukin-1 receptor-associated kinase (IRAK) family members. Mol. Cell. 2003; 11:293-302.
Background Reference 4:
Sato S, Sugiyama M, Yamamoto M, et al. Toll/IL-1 receptor domain-containing adaptor inducing IFN-β (TRIF) associates with TNF receptor-associated factor 6 and TANK-binding kinase 1, and activates two distinct transcription factors, NF-κ B and IFN-regulatory factor-3, in the Toll-like receptor signaling. J. Immunol. 2003; 171(8):4304-10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3377

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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