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43-429 (1ug/ml) staining of Human lung (A) and Human Umbilical Cord (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour.
43-429 (5ug/ml) staining of paraffin embedded Human Vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-429 (1ug/ml) staining of Human lung (A) and Human Umbilical Cord (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour.

ECSM2 Antibody

CATALOG NUMBER: 43-429

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-NNGKQSLSAEKVL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Lung and Human Umbilical Cord lysates (calculated MW of 21.3kDa according to NP_001071161.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ref PMID: 18556573). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Vessel. Recommended concentration: 5ug/ml. This product has been successfully used in IHC on Human: Matsuo K et al, J Biol Chem. 2015 Feb 6;290(6):3784. PMID: 25533470.Immunofluorescence: This product has been successfully used in IF on Human: Matsuo K et al, J Biol Chem. 2015 Feb 6;290(6):3784. PMID: 25533470.
Predicted Molecular Weight:
Approx 50kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ECSM2
Additional Names:
endothelial cell-specific marker 2, endothelial cell-specific molecule 2, hypothetical protein LOC641700
Protein Accession Number:
NP_001071161.1
Protein Gi Number:
118130809
Ncbi Gene Id Number:
641700

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-429

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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